LOCUS       PBR322       4363 bp ds-DNA   circular   SYN       15-DEC-1988
DEFINITION  Plasmid pBR322, complete genome.
ACCESSION   J01749 K00005 M10282 M10283 M10286 M10356 M10784 M10785 M10786
SOURCE  
  ORGANISM  Cloning vector
REFERENCE   1
  AUTHOR    Sutcliffe,J.G.
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 43, 77-90 (1979)
 STANDARD   full      
REFERENCE   2
  AUTHOR    Sutcliffe,J.G.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 75, 3737-3741 (1978)
REFERENCE   3
  AUTHOR    Reed,R.R., Young,R.A., Steitz,J.A., Grindley,N.D.F. and Guyer,M.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 76, 4882-4886 (1979)
REFERENCE   4
  AUTHOR    Covarrubias,L., Cervantes,L., Covarrubias,A., Soberon,X., 
            Vichido,I., Blanco,A., Kupersztoch-Portnoy,Y.M. and Bolivar,F.
  JOURNAL   Gene 13, 25-35 (1981)
REFERENCE   5
  AUTHOR    Brosius,J., Cate,R.L. and Perlmutter,A.P.
  JOURNAL   J. Biol. Chem. 257, 9205-9210 (1982)
REFERENCE   6
  AUTHOR    Livneh,Z.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80, 237-241 (1983)
REFERENCE   7
  AUTHOR    Peden,K.W.C. and Nathans,D.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 79, 7214-7217 (1982)
REFERENCE   8
  AUTHOR    Marians,K.J., Soeller,W. and Zipursky,S.L.
  JOURNAL   J. Biol. Chem. 257, 5656-5662 (1982)
REFERENCE   9
  AUTHOR    Van Dyke,M.W., Hertzberg,R.P. and Dervan,P.B.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 79, 5470-5474 (1982)
REFERENCE   10
  AUTHOR    Mascharak,P.K., Sugiura,Y., Kuwahara,J., Suzuki,T. and 
            Lippard,S.J.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80, 6795-6798 (1983)
REFERENCE   11
  AUTHOR    Schultz,P.G. and Dervan,P.B.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80, 6834-6837 (1983)
REFERENCE   12
  AUTHOR    Watabe,H.-O., Iino,T., Kaneko,T., Shibata,T. and Ando,T.
  JOURNAL   J. Biol. Chem. 258, 4663-4665 (1983)
REFERENCE   13
  AUTHOR    Peden,K.W.C.
  JOURNAL   Gene 22, 277-280 (1983)
REFERENCE   14
  AUTHOR    Sutcliffe,J.G.
  JOURNAL   Unpublished (1984) personal communication
REFERENCE   15
  AUTHOR    Abarzua,P., Soeller,W. and Marians,K.J.
  JOURNAL   J. Biol. Chem. 259, 14286-14292 (1984)
REFERENCE   16
  AUTHOR    Soeller,W., Abarzua,P. and Marians,K.J.
  JOURNAL   J. Biol. Chem. 259, 14293-14300 (1984)
REFERENCE   17
  AUTHOR    Fuller,R.S., Funnell,B.E. and Kornberg,A.
  JOURNAL   Cell 38, 889-900 (1984)
REFERENCE   18
  AUTHOR    Van Dyke,M.M. and Dervan,P.B.
  JOURNAL   Science 225, 1122-1127 (1984)
REFERENCE   19
  AUTHOR    Lathe,R., Kieny,M.P., Skory,S. and Lecocoq,J.P.
  JOURNAL   DNA 3, 173-182 (1984)
REFERENCE   20
  AUTHOR    Huesterspreute,M. and Davison,J.
  JOURNAL   DNA 3, 259-268 (1984)
FEATURES       from  to/span     description
    pept         86     1276     tetracycline resistance peptide
    pept       4155     3295      (c) beta-lactamase
    sigp       4152     4087      (c) beta-lactamase signal peptide
    matp       4086     3298      (c) beta-lactamase mature peptide
    binding      24       27     echinomycin binding site tcga [18]
    binding      39       42     echinomycin binding site gcgg [18]
    binding      53       56     echinomycin binding site cagt [18]
    binding      67       70     echinomycin binding site acgc [18]
    binding      80       83     echinomycin binding site ccgt [18]
    binding     411      414     echinomycin binding site ccgg [18]
    binding     469      472     echinomycin binding site tcgg [18]
    binding    2441     2449     dnaA binding site
    binding    4273     4270      (c) echinomycin binding site tcgg [18]
    binding    4285     4282      (c) echinomycin binding site aggt [18]
    binding    4290     4287      (c) echinomycin binding site acgt [18]
    binding    4301     4298      (c) echinomycin binding site tggt [18]
    binding    4316     4313      (c) echinomycin binding site atgt [18]
    binding    4322     4319      (c) echinomycin binding site aggt [18]
    binding    4336     4333      (c) echinomycin binding site acgc [18]
    site       1519     1515      (c) gamma-delta insertion target sequence [3]
    site       1792     1788      (c) gamma-delta insertion target sequence [3]
    site       2251     2247      (c) gamma-delta insertion target sequence [3]
    site       2013     2169     H-strand Y effector site [8]
    site       2416     2353      (c) L-strand Y effector site [8]
    signal       33       27      (c) promoter P1 [5]
    signal       43       49     promoter P2 [5]
    signal     4196     4190      (c) promoter P3 [5]
    attack      142      141      (c) Endo.Sce I cleavage site coordinated with
                                 site at base 146 [12]
    attack      146      147     Endo.Sce I cleavage site coordinated with
                                 site at base 142 [12]
    orgrpl     2537     2537     replication origin
    conflict    426      426     c in [1]; a in [6]
    refnumbr      1        1     numbered 1 in [1],[5]
    refnumbr    134      134     numbered 134 in [12]
    refnumbr    368      368     numbered 368 in [6]
    refnumbr    375      375     numbered 375 in [13]
    refnumbr    520      520     sequence not numbered in [14]
    revision    526      528     ccc in [13]; cc in [1]
    refnumbr    584      584     sequence not numbered in [7]
    refnumbr   2115     2115     numbered 1 in [8]
    refnumbr   2209     2209     sequence not numbered in [4]
    refnumbr   2397     2397     sequence not numbered in [17]
    refnumbr   2417     2417     numbered 2416 in [15],[16]
    refnumbr   2684     2684      (c) numbered 1 in [10]
    revision   2731     2731     t in [18]; a in [1]
    revision   2732     2732     a in [19]; t in [1]
    refnumbr   4338     4338      (c) sequence not numbered in [11]
    refnumbr   4345     4345      (c) sequence not numbered in [9]
    refnumbr   4347     4347     sequence not numbered in [18]
    refnumbr   4363     4363      (c) numbered 1 in [2]
BASE COUNT      984 a   1210 c   1134 g   1035 t
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg 
     1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg 
     1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc 
     1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg 
     1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca 
     1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga 
     1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa 
     1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt 
     1861 ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg catcaagtga 
     1921 ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc 
     1981 ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc 
     2041 acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa 
     2101 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 
     2161 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 
     2221 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat 
     2281 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 
     2341 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     2401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     2461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     2521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     2581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     2641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     2701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     2761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     2821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     2881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     2941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
     3001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     3061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     3121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     3181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     3241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     3301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     3361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     3421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     3481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     3541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     3601 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     3661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     3721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     3781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     3841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     3901 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     3961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     4021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     4081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     4141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     4201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     4261 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 
     4321 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaa
//
